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Soil Metadata Survey for Microbial Studies

The purpose of this survey is to determine what data about soil is believed to be important to accompany microbial molecular data entered into major databases, e.g GenBank, EMBL, RDP. The value of this metadata is to enable a search of the data for particular features and for determining relationships among soil features and molecular data over more global data sets. We are surveying the soil biology community to gain this assessment and then will work with the Genomic Standards Consortium (GSC) and the Environmental Ontology Subgroup to ensure that soil data will be properly considered along with the metadata from other environments.

--from Jim Tiedje, Michigan State University, as an initiative
of the Genomic Standards Consortium and the new
International Soil Metagenome Consortium (Terragenome)




INSTRUCTIONS: Click a 1, 2, 3, 4 before each characteristic based on your opinion. The sets are categorized by:

Importance
(to the understanding of microbial ecology)

  • 1 = Not important at all
  • 2 = Minimally important
  • 3 = Moderately important
  • 4 = Very important
AND

soil-sampling

Difficulty
(how burdensome is collecting this data/
are you likely to provide it)

  • 1 = Easy to obtain
  • 2 = Moderately easy
  • 3 = Moderately difficult
  • 4 = Very difficult to obtain


Click the checkbox for items you believe should be "required" (to accompany all molecular data entry; minimum metadata set).


Chemical

Importance Difficulty Req'd



pH (in water or Calcium chloride)



salinity/conductivity



total organic C



total N



nitrogen species (e.g., Nitrate, Ammonia)



total P



total S



Ca, Mg



carbonates



available carbon (e.g., DOC...)



carbon fractions (e.g., light, heavy, occluded, fulvic, humic acids)



mineralogy



pore space oxygen concentration



Eh (redox potential)



cation exchange capacity



base saturation



Al saturation



pollutants (e.g., solvents, heavy metals)



trace elements (e.g., metals:Fe, Mn, Cu...)




Physical

Importance Difficulty Req'd



texture (granulometry)



aggregate stability



penetration resistance



soil structure



porosity



bulk density



water retention curve



field capacity



wilting coeffecient



infiltration capacity



slope, position




Biological

Importance Difficulty Req'd



microbial biomass and its C,N,P



C/N ratio



respiration rate



N cycle rates (e.g., nitrification)



plant cover (native)



root biomass



enzymatic activities (e.g., dehydrogenases)



annual primary productivity of site



annual plant residue returned to soil



soil fauna (types, density)



earthworm activity or density



susceptibility to microbe immigration (water, dust, birds…)




Soil / Geological

Importance Difficulty Req'd



soil type (classification)



horizon



drainage classification



parent rock material



water table (maximum and minimum annual depths)



degree of heterogeneity within sampled site




Geographical

Importance Difficulty Req'd



latitude and longitude



elevation




Management

Importance Difficulty Req'd



land use (e.g., urban, agriculture, forestry)



tillage (type)



crops (current, rotation)



crop yield



animal activity (e.g., feral pigs, grazing)



fertilizers (type and annual amount)



pesticides



historical data (e.g., previous land use)



fire



erosion




Climate

Importance Difficulty Req'd



mean and seasonal rainfall



mean and seasonal temperatures



evapotransportation



global radiation




Sampling

Importance Difficulty Req'd



depth



composite design



soil temperature at sampling



weather at sampling



moisture content at sampling



area represented by composite sample



weight of sample used for DNA extraction






Additional comments: